黔南州核桃优株立地土壤养分因子分析

为了指导黔南州核桃园土壤管理与合理施肥,应用SPSS统计分析软件对黔南州的核桃优株立地土壤养分因子进行了相关分析和主成分分析。结果表明:土壤养分各指标间存在明显的相关关系;由主成分分析提取出的3个主成分可全面概括9个土壤养分指标;土壤养分指标综合得分最高的是罗甸-2号核桃。黔南州核桃优株间立地土壤养分状况存在明显差异。     

贵州甘薯地方品种的生长分析

以甘薯品种徐薯18为对照(CK),采用生长分析法,通过田间试验,对贵州6个甘薯地方品种的生长性能进行了分析。结果表明:品种"道真白皮苕"的块根和茎叶鲜重明显高于CK的;品种"道真白皮苕"、"紫云红心薯"的干物质积累量、LAI、RGR及NAR均明显高于CK的。因此在供试的6个甘薯地方品种中,道真白皮苕和紫云红心薯的物质生产性能较优。     

手扶式田间电动运输车的虚拟样机设计

研究了一种手扶式田间烟叶电动运输车,采用轮毂电机驱动,整车结构精简、轻巧,能够大大提高丘陵地区田间的运输效率及安全性,降低劳动强度。采用UG三维制图及有限元分析方法,对车体关键零部件进行三维造型,并对整车关键零部件前后轴进行了应力应变分析,优化了设计方案。田间道路试验表明整车结构设计合理,满足性能要求。     

百里杜鹃林区采煤塌陷前后土壤养分状况对比研究

【目的】揭示采煤塌陷对贵州百里杜鹃林区土壤养分的影响。[方法】在选取典型对比样地的基础上,对百里杜鹃林区不同层次土壤进行了分层对比分析。【结果]结果表明,采煤塌陷后,百里杜鹃林区土壤酸性减弱,向中性趋近;土壤有机质含量有降低的趋势;土壤全量养分（全氮、全磷、全钾）含量有所提高;有效氮（铵态氮、硝态氮）含量总体提高,但速效磷、速效钾含量明显降低。[结论]采煤塌陷打破了百里杜鹃林区土壤养分的平衡,尤其是减少了有效磷、有效钾的供应。     

低压电源在口腔技工桌中的应用研究

指出了由于加热器械的使用,各种电源线易被烫坏,电源发生短路,使得口腔修复实训室用电存在严重的安全隐患。研究了通过将口腔技工桌设计为24V低压供电,减少口腔技工桌上的电源线的数量,以避免引发人身触电事故,保证了口腔修复实训用电安全,同时可以直接使用清洁的太阳能及风能,达到节能、环保、安全的目的。     

提高威宁县马铃薯脱毒种薯质量的关键技术

从选种、播种密度、播种时间、去杂、蚜虫防治、种薯质量检测和适时收获等方面阐述了提高威宁县马铃薯种薯质量的关键技术。     

贵州省白及叶褐斑病病原菌鉴定及其生物学特性

为明确贵州省白及叶褐斑病病原菌及其生物学特性,采用组织分离法和离体接种法分别对其病原菌进行分离和致病性测定,利用形态学及ITS、EF-1α、β-tubulin基因序列分析对病原菌的分类进行鉴定,并对病原菌的生物学特性进行研究。结果表明:分离得到的白及叶褐斑病病原菌菌株编号为HGUP_(17)355;该菌菌丝白色、羊绒状,菌落中心隆起呈淡黄色、背面枫叶红色,大型分生孢子窄镰刀形,3～8个分隔,未见厚垣孢子,小型分生孢子卵圆形,1～2个分隔;在以ITS、EF-1α、β-tubulin三个基因序列构建的系统发育树中,菌株HGUP_(17)355与燕麦镰刀菌Fusarium avenaceum聚于一支,且支持率达100%,结合形态特征与分子生物学将其鉴定为燕麦镰刀菌。该菌菌丝最适生长温度为20℃,最适生长pH为6,在燕麦片琼脂培养基上生长最快,以麦芽糖为碳源、以L-苯丙氨酸为氮源时利用率最高,致死温度为45℃,对光照不敏感。     

Extraction,Purification and Activity Analysis of Lipopolysaccharides from Escherichia coli Isolated from Swine

In order to obtain higher purity and high yield of LPS isolated from Escherichia coli, and evaluate its virulence,excrement of diarrhea piglet was collected as pathological samples, of which, some strains of Escherichia coli were isolated and identified. And furthermore, a strain of Escherichia coli was acquired by 16S rDNA identification. The LPS from this strain of Escherichia coli was isolated with the hot-phenol water method and purified by DNaseⅠ, RNaseA, proteinase K treatment and alcohol precipitation. The polysaccharide, protein and nucleic acid content of purified LPS were detected by phenol-sulfuric acid method, Bradford method and UV points spectrophotometric method. And its bioactivity and acute median lethal dose was determinated by tachypleus amebocyte lysate (TAL) method and acute toxicity experiment method, respectively. The results showed that a strain of highly pathogenic Escherichia coli was successfully isolated. The average yield of purified LPS was 3.74%, the proportion of the polysaccharide was 13.40%, the protein was 0.48%, and the nucleic acid was 0.06%. SDS-PAGE electrophoresis and silver stain showed that the bands were mainly concentrated in the range of 14 to 160 ku. The LAL bioactivity of the prepared LPS was 1.21×10⁷ EU/mg, LD₅₀ of the mouse abdominal cavity was 31.86 mg/kg. The results revealed that the diarrhea piglets in the farm was caused by pathogenic Escherichia coli,purified LPS from this strain of Escherichia coli had the advantages of higher purity, high yield and strong virulence, which would provide theoretical data for clinical prevention and treatment of diarrhea piglets.     

芭蕉芋的种质资源及栽培技术研究进展

为了推进芭蕉芋(Canna edulis Ker)的生产和发展,国内外学者对芭蕉芋的种植进行了深入系统的研究。在此基础上,对近年来国内外关于芭蕉芋种质资源、营养成分及用途、生理特性和栽培技术进行了综述,并针对芭蕉芋的研究进展展望了今后的研究方向。